Abstract
The effect of UV-B on the occurrence of DNA damage and consequences for the cell cycle were studied in the marine diatom Cyclotella sp. DNA damage was quantified by immunofluorescent detection of thymine dimers in nuclear DNA of single cells using flow cytometry. A total UV-B dose (biologically effective dose BE(DNA300nm)) of 1.05 kJ m(-2) caused detectable damage in about 20% of the exposed population. In the period after the UV-B treatment, when the culture was exposed to PAR (450 mu mol m(-2)s(-1)) only, thymine dimers were removed; after 8 hours none of these photoproducts remained. Cellular DNA content measurements and quantification of the fraction of recently divided cells revealed that the DNA synthesis rate as well as the rate of cell division were reduced during and shortly after UV-B exposure. Apparently, UV-B irradiation extends the cell cycle of Cyclotella sp. in the S (DNA synthesis) phase until the dimers are removed. [KEYWORDS: cell cycle; DNA damage; flow-cytometry; marine diatom; thymine dimers; UV-B Ultraviolet-radiation; antarctica; dosimeter; dimers; ocean;cells]
Original language | English |
---|---|
Pages (from-to) | 101-106 |
Journal | Scientia Marina |
Volume | 60 |
Publication status | Published - 1996 |
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Buma, A. G. J., Van Hannen, E. J., Veldhuis, M., & Gieskes, W. W. C. (1996). UV-B induces DNA damage and DNA synthesis delay in the marine diatom Cyclotella sp. Scientia Marina, 60, 101-106. http://www.icm.csic.es/scimar/index.php/secId/6/IdArt/359/
Buma, A.G.J. ; Van Hannen, E.J. ; Veldhuis, M. et al. / UV-B induces DNA damage and DNA synthesis delay in the marine diatom Cyclotella sp. In: Scientia Marina. 1996 ; Vol. 60. pp. 101-106.
@article{ec06ff16965240c6853993f6bf9142aa,
title = "UV-B induces DNA damage and DNA synthesis delay in the marine diatom Cyclotella sp",
abstract = "The effect of UV-B on the occurrence of DNA damage and consequences for the cell cycle were studied in the marine diatom Cyclotella sp. DNA damage was quantified by immunofluorescent detection of thymine dimers in nuclear DNA of single cells using flow cytometry. A total UV-B dose (biologically effective dose BE(DNA300nm)) of 1.05 kJ m(-2) caused detectable damage in about 20% of the exposed population. In the period after the UV-B treatment, when the culture was exposed to PAR (450 mu mol m(-2)s(-1)) only, thymine dimers were removed; after 8 hours none of these photoproducts remained. Cellular DNA content measurements and quantification of the fraction of recently divided cells revealed that the DNA synthesis rate as well as the rate of cell division were reduced during and shortly after UV-B exposure. Apparently, UV-B irradiation extends the cell cycle of Cyclotella sp. in the S (DNA synthesis) phase until the dimers are removed. [KEYWORDS: cell cycle; DNA damage; flow-cytometry; marine diatom; thymine dimers; UV-B Ultraviolet-radiation; antarctica; dosimeter; dimers; ocean;cells]",
author = "A.G.J. Buma and {Van Hannen}, E.J. and M. Veldhuis and W.W.C. Gieskes",
note = "Reporting year: 1996 Metis note: 2959; CL; AFW; file:///L:/Endnotedatabases/NIOOPUB/pdfs/Pdfs1996/Buma_ea_2959.pdf",
year = "1996",
language = "English",
volume = "60",
pages = "101--106",
journal = "Scientia Marina",
issn = "0214-8358",
publisher = "Consejo Superior de Investigaciones Cientificas (CSIC)",
}
Buma, AGJ, Van Hannen, EJ, Veldhuis, M & Gieskes, WWC 1996, 'UV-B induces DNA damage and DNA synthesis delay in the marine diatom Cyclotella sp', Scientia Marina, vol. 60, pp. 101-106. <http://www.icm.csic.es/scimar/index.php/secId/6/IdArt/359/>
UV-B induces DNA damage and DNA synthesis delay in the marine diatom Cyclotella sp. / Buma, A.G.J.; Van Hannen, E.J.; Veldhuis, M. et al.
In: Scientia Marina, Vol. 60, 1996, p. 101-106.
Research output: Contribution to journal/periodical › Article › Scientific › peer-review
TY - JOUR
T1 - UV-B induces DNA damage and DNA synthesis delay in the marine diatom Cyclotella sp
AU - Buma, A.G.J.
AU - Van Hannen, E.J.
AU - Veldhuis, M.
AU - Gieskes, W.W.C.
N1 - Reporting year: 1996Metis note: 2959; CL; AFW; file:///L:/Endnotedatabases/NIOOPUB/pdfs/Pdfs1996/Buma_ea_2959.pdf
PY - 1996
Y1 - 1996
N2 - The effect of UV-B on the occurrence of DNA damage and consequences for the cell cycle were studied in the marine diatom Cyclotella sp. DNA damage was quantified by immunofluorescent detection of thymine dimers in nuclear DNA of single cells using flow cytometry. A total UV-B dose (biologically effective dose BE(DNA300nm)) of 1.05 kJ m(-2) caused detectable damage in about 20% of the exposed population. In the period after the UV-B treatment, when the culture was exposed to PAR (450 mu mol m(-2)s(-1)) only, thymine dimers were removed; after 8 hours none of these photoproducts remained. Cellular DNA content measurements and quantification of the fraction of recently divided cells revealed that the DNA synthesis rate as well as the rate of cell division were reduced during and shortly after UV-B exposure. Apparently, UV-B irradiation extends the cell cycle of Cyclotella sp. in the S (DNA synthesis) phase until the dimers are removed. [KEYWORDS: cell cycle; DNA damage; flow-cytometry; marine diatom; thymine dimers; UV-B Ultraviolet-radiation; antarctica; dosimeter; dimers; ocean;cells]
AB - The effect of UV-B on the occurrence of DNA damage and consequences for the cell cycle were studied in the marine diatom Cyclotella sp. DNA damage was quantified by immunofluorescent detection of thymine dimers in nuclear DNA of single cells using flow cytometry. A total UV-B dose (biologically effective dose BE(DNA300nm)) of 1.05 kJ m(-2) caused detectable damage in about 20% of the exposed population. In the period after the UV-B treatment, when the culture was exposed to PAR (450 mu mol m(-2)s(-1)) only, thymine dimers were removed; after 8 hours none of these photoproducts remained. Cellular DNA content measurements and quantification of the fraction of recently divided cells revealed that the DNA synthesis rate as well as the rate of cell division were reduced during and shortly after UV-B exposure. Apparently, UV-B irradiation extends the cell cycle of Cyclotella sp. in the S (DNA synthesis) phase until the dimers are removed. [KEYWORDS: cell cycle; DNA damage; flow-cytometry; marine diatom; thymine dimers; UV-B Ultraviolet-radiation; antarctica; dosimeter; dimers; ocean;cells]
M3 - Article
SN - 0214-8358
VL - 60
SP - 101
EP - 106
JO - Scientia Marina
JF - Scientia Marina
ER -
Buma AGJ, Van Hannen EJ, Veldhuis M, Gieskes WWC. UV-B induces DNA damage and DNA synthesis delay in the marine diatom Cyclotella sp. Scientia Marina. 1996;60:101-106.