Monitoring Ultraviolet-B-Induced DNA-Damage in Individual Diatom Cells by Immunofluorescent Thymine Dimer Detection (2024)

Buma, A. G. J., Van Hannen, E. J., Roza, L., Veldhuis, M., & Gieskes, W. W. C. (1995). Monitoring Ultraviolet-B-Induced DNA-Damage in Individual Diatom Cells by Immunofluorescent Thymine Dimer Detection. Journal of Phycology, 31(2), 314-321. https://doi.org/10.1111/j.0022-3646.1995.00314.x

Buma, A.G.J. ; Van Hannen, E.J. ; Roza, L. et al. / Monitoring Ultraviolet-B-Induced DNA-Damage in Individual Diatom Cells by Immunofluorescent Thymine Dimer Detection. In: Journal of Phycology. 1995 ; Vol. 31, No. 2. pp. 314-321.

@article{9905216d37a944e789ab5cc147c8ff48,

title = "Monitoring Ultraviolet-B-Induced DNA-Damage in Individual Diatom Cells by Immunofluorescent Thymine Dimer Detection",

abstract = "We developed a method to investigate the effect of ultraviolet- B radiation (UVBR) on the formation of thymine dimers in microalgal DIVA that can be used for both laboratory and in situ research. Antibody labeling of dimers was followed by a secondary antibody (fluorescein isothiocyanate) staining to allow visualization of DNA damage with flow cytometry or fluorescence microscopy. Thymine dimer-specific fluorescence in nuclear DNA of the marine diatom Cyclotella sp. was linearly related to the UVBR dose. Simultaneous measurements of cellular DNA content showed that the vulnerability of G2 cells to DNA damage did not differ significantly from the vulnerability of G1 cells. The formation and removal of thymine dimers in Cyclotella sp. cells was monitored for 3 consecutive days at two realistic UVBR irradiance bevels. Thymine dimers were removed within 24 h when exposed to a saturating photosynthetically active radiation intensity following the UVBR treatment. This new method allows the study of UVBR- induced DNA damage on a cell-to-cell basis. It is also feasible for field studies because cells remain intact and can be recognized readily after antibody treatment. [KEYWORDS: Bacillariophyceae; cyclotella; dna damage; monoclonal antibody; thymine dimers; uv-b Phytoplankton photosynthesis; radiation; repair; fibroblasts; inhibition; antarctica; dosimeter; growth; impact; ocean]",

author = "A.G.J. Buma and {Van Hannen}, E.J. and L. Roza and M. Veldhuis and W.W.C. Gieskes",

note = "Reporting year: 1995 Metis note: 2022; CL; AFW; file:///L:/Endnotedatabases/NIOOPUB/pdfs/Pdfs1995/Buma_ea_2022.pdf",

year = "1995",

doi = "10.1111/j.0022-3646.1995.00314.x",

language = "English",

volume = "31",

pages = "314--321",

journal = "Journal of Phycology",

issn = "0022-3646",

publisher = "Wiley-Blackwell",

number = "2",

}

Buma, AGJ, Van Hannen, EJ, Roza, L, Veldhuis, M & Gieskes, WWC 1995, 'Monitoring Ultraviolet-B-Induced DNA-Damage in Individual Diatom Cells by Immunofluorescent Thymine Dimer Detection', Journal of Phycology, vol. 31, no. 2, pp. 314-321. https://doi.org/10.1111/j.0022-3646.1995.00314.x

Monitoring Ultraviolet-B-Induced DNA-Damage in Individual Diatom Cells by Immunofluorescent Thymine Dimer Detection. / Buma, A.G.J.; Van Hannen, E.J.; Roza, L. et al.
In: Journal of Phycology, Vol. 31, No. 2, 1995, p. 314-321.

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

TY - JOUR

T1 - Monitoring Ultraviolet-B-Induced DNA-Damage in Individual Diatom Cells by Immunofluorescent Thymine Dimer Detection

AU - Buma, A.G.J.

AU - Van Hannen, E.J.

AU - Roza, L.

AU - Veldhuis, M.

AU - Gieskes, W.W.C.

N1 - Reporting year: 1995Metis note: 2022; CL; AFW; file:///L:/Endnotedatabases/NIOOPUB/pdfs/Pdfs1995/Buma_ea_2022.pdf

PY - 1995

Y1 - 1995

N2 - We developed a method to investigate the effect of ultraviolet- B radiation (UVBR) on the formation of thymine dimers in microalgal DIVA that can be used for both laboratory and in situ research. Antibody labeling of dimers was followed by a secondary antibody (fluorescein isothiocyanate) staining to allow visualization of DNA damage with flow cytometry or fluorescence microscopy. Thymine dimer-specific fluorescence in nuclear DNA of the marine diatom Cyclotella sp. was linearly related to the UVBR dose. Simultaneous measurements of cellular DNA content showed that the vulnerability of G2 cells to DNA damage did not differ significantly from the vulnerability of G1 cells. The formation and removal of thymine dimers in Cyclotella sp. cells was monitored for 3 consecutive days at two realistic UVBR irradiance bevels. Thymine dimers were removed within 24 h when exposed to a saturating photosynthetically active radiation intensity following the UVBR treatment. This new method allows the study of UVBR- induced DNA damage on a cell-to-cell basis. It is also feasible for field studies because cells remain intact and can be recognized readily after antibody treatment. [KEYWORDS: Bacillariophyceae; cyclotella; dna damage; monoclonal antibody; thymine dimers; uv-b Phytoplankton photosynthesis; radiation; repair; fibroblasts; inhibition; antarctica; dosimeter; growth; impact; ocean]

AB - We developed a method to investigate the effect of ultraviolet- B radiation (UVBR) on the formation of thymine dimers in microalgal DIVA that can be used for both laboratory and in situ research. Antibody labeling of dimers was followed by a secondary antibody (fluorescein isothiocyanate) staining to allow visualization of DNA damage with flow cytometry or fluorescence microscopy. Thymine dimer-specific fluorescence in nuclear DNA of the marine diatom Cyclotella sp. was linearly related to the UVBR dose. Simultaneous measurements of cellular DNA content showed that the vulnerability of G2 cells to DNA damage did not differ significantly from the vulnerability of G1 cells. The formation and removal of thymine dimers in Cyclotella sp. cells was monitored for 3 consecutive days at two realistic UVBR irradiance bevels. Thymine dimers were removed within 24 h when exposed to a saturating photosynthetically active radiation intensity following the UVBR treatment. This new method allows the study of UVBR- induced DNA damage on a cell-to-cell basis. It is also feasible for field studies because cells remain intact and can be recognized readily after antibody treatment. [KEYWORDS: Bacillariophyceae; cyclotella; dna damage; monoclonal antibody; thymine dimers; uv-b Phytoplankton photosynthesis; radiation; repair; fibroblasts; inhibition; antarctica; dosimeter; growth; impact; ocean]

U2 - 10.1111/j.0022-3646.1995.00314.x

DO - 10.1111/j.0022-3646.1995.00314.x

M3 - Article

SN - 0022-3646

VL - 31

SP - 314

EP - 321

JO - Journal of Phycology

JF - Journal of Phycology

IS - 2

ER -

Buma AGJ, Van Hannen EJ, Roza L, Veldhuis M, Gieskes WWC. Monitoring Ultraviolet-B-Induced DNA-Damage in Individual Diatom Cells by Immunofluorescent Thymine Dimer Detection. Journal of Phycology. 1995;31(2):314-321. doi: 10.1111/j.0022-3646.1995.00314.x