Monitoring Ultraviolet-B-Induced DNA-damage in Individual Diatom Cells by Immunofluorescent Thymine Dimer Detection (2024)

Buma, A. G. J., Van Hannen, E. J., Roza, L., Veldhuis, M. J. W., & Gieskes, W. W. C. (1995). Monitoring Ultraviolet-B-Induced DNA-damage in Individual Diatom Cells by Immunofluorescent Thymine Dimer Detection. Journal of Phycology, 31(2), 314-321. https://doi.org/10.1111/j.0022-3646.1995.00314.x

Buma, Anita G. J. ; Van Hannen, Erik J. ; Roza, Len et al. / Monitoring Ultraviolet-B-Induced DNA-damage in Individual Diatom Cells by Immunofluorescent Thymine Dimer Detection. In: Journal of Phycology. 1995 ; Vol. 31, No. 2. pp. 314-321.

@article{e80fa9d8bd294c54b2f33336ba8b8960,

title = "Monitoring Ultraviolet-B-Induced DNA-damage in Individual Diatom Cells by Immunofluorescent Thymine Dimer Detection",

abstract = "We developed a method to investigate the effect of ultraviolet-B radiation (UVBR) on the formation of thymine dimers in microalgal DIVA that can be used for both laboratory and in situ research. Antibody labeling of dimers was followed by a secondary antibody (fluorescein isothiocyanate) staining to allow visualization of DNA damage with flow cytometry or fluorescence microscopy. Thymine dimer-specific fluorescence in nuclear DNA of the marine diatom Cyclotella sp. was linearly related to the UVBR dose. Simultaneous measurements of cellular DNA content showed that the vulnerability of G2 cells to DNA damage did not differ significantly from the vulnerability of G1 cells. The formation and removal of thymine dimers in Cyclotella sp. cells was monitored for 3 consecutive days at two realistic UVBR irradiance bevels. Thymine dimers were removed within 24 h when exposed to a saturating photosynthetically active radiation intensity following the UVBR treatment. This new method allows the study of UVBR-induced DNA damage on a cell-to-cell basis. It is also feasible for field studies because cells remain intact and can be recognized readily after antibody treatment.",

keywords = "BACILLARIOPHYCEAE, CYCLOTELLA, DNA DAMAGE, MONOCLONAL ANTIBODY, THYMINE DIMERS, UV-B, PHYTOPLANKTON PHOTOSYNTHESIS, RADIATION, REPAIR, FIBROBLASTS, INHIBITION, ANTARCTICA, DOSIMETER, GROWTH, IMPACT, OCEAN",

author = "Buma, {Anita G. J.} and {Van Hannen}, {Erik J.} and Len Roza and Veldhuis, {Marcel J. W.} and Gieskes, {Winfried W. C.}",

year = "1995",

month = apr,

doi = "10.1111/j.0022-3646.1995.00314.x",

language = "English",

volume = "31",

pages = "314--321",

journal = "Journal of Phycology",

issn = "0022-3646",

publisher = "Wiley",

number = "2",

}

Buma, AGJ, Van Hannen, EJ, Roza, L, Veldhuis, MJW & Gieskes, WWC 1995, 'Monitoring Ultraviolet-B-Induced DNA-damage in Individual Diatom Cells by Immunofluorescent Thymine Dimer Detection', Journal of Phycology, vol. 31, no. 2, pp. 314-321. https://doi.org/10.1111/j.0022-3646.1995.00314.x

Monitoring Ultraviolet-B-Induced DNA-damage in Individual Diatom Cells by Immunofluorescent Thymine Dimer Detection. / Buma, Anita G. J.; Van Hannen, Erik J.; Roza, Len et al.
In: Journal of Phycology, Vol. 31, No. 2, 04.1995, p. 314-321.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Monitoring Ultraviolet-B-Induced DNA-damage in Individual Diatom Cells by Immunofluorescent Thymine Dimer Detection

AU - Buma, Anita G. J.

AU - Van Hannen, Erik J.

AU - Roza, Len

AU - Veldhuis, Marcel J. W.

AU - Gieskes, Winfried W. C.

PY - 1995/4

Y1 - 1995/4

N2 - We developed a method to investigate the effect of ultraviolet-B radiation (UVBR) on the formation of thymine dimers in microalgal DIVA that can be used for both laboratory and in situ research. Antibody labeling of dimers was followed by a secondary antibody (fluorescein isothiocyanate) staining to allow visualization of DNA damage with flow cytometry or fluorescence microscopy. Thymine dimer-specific fluorescence in nuclear DNA of the marine diatom Cyclotella sp. was linearly related to the UVBR dose. Simultaneous measurements of cellular DNA content showed that the vulnerability of G2 cells to DNA damage did not differ significantly from the vulnerability of G1 cells. The formation and removal of thymine dimers in Cyclotella sp. cells was monitored for 3 consecutive days at two realistic UVBR irradiance bevels. Thymine dimers were removed within 24 h when exposed to a saturating photosynthetically active radiation intensity following the UVBR treatment. This new method allows the study of UVBR-induced DNA damage on a cell-to-cell basis. It is also feasible for field studies because cells remain intact and can be recognized readily after antibody treatment.

AB - We developed a method to investigate the effect of ultraviolet-B radiation (UVBR) on the formation of thymine dimers in microalgal DIVA that can be used for both laboratory and in situ research. Antibody labeling of dimers was followed by a secondary antibody (fluorescein isothiocyanate) staining to allow visualization of DNA damage with flow cytometry or fluorescence microscopy. Thymine dimer-specific fluorescence in nuclear DNA of the marine diatom Cyclotella sp. was linearly related to the UVBR dose. Simultaneous measurements of cellular DNA content showed that the vulnerability of G2 cells to DNA damage did not differ significantly from the vulnerability of G1 cells. The formation and removal of thymine dimers in Cyclotella sp. cells was monitored for 3 consecutive days at two realistic UVBR irradiance bevels. Thymine dimers were removed within 24 h when exposed to a saturating photosynthetically active radiation intensity following the UVBR treatment. This new method allows the study of UVBR-induced DNA damage on a cell-to-cell basis. It is also feasible for field studies because cells remain intact and can be recognized readily after antibody treatment.

KW - BACILLARIOPHYCEAE

KW - CYCLOTELLA

KW - DNA DAMAGE

KW - MONOCLONAL ANTIBODY

KW - THYMINE DIMERS

KW - UV-B

KW - PHYTOPLANKTON PHOTOSYNTHESIS

KW - RADIATION

KW - REPAIR

KW - FIBROBLASTS

KW - INHIBITION

KW - ANTARCTICA

KW - DOSIMETER

KW - GROWTH

KW - IMPACT

KW - OCEAN

U2 - 10.1111/j.0022-3646.1995.00314.x

DO - 10.1111/j.0022-3646.1995.00314.x

M3 - Article

SN - 0022-3646

VL - 31

SP - 314

EP - 321

JO - Journal of Phycology

JF - Journal of Phycology

IS - 2

ER -

Buma AGJ, Van Hannen EJ, Roza L, Veldhuis MJW, Gieskes WWC. Monitoring Ultraviolet-B-Induced DNA-damage in Individual Diatom Cells by Immunofluorescent Thymine Dimer Detection. Journal of Phycology. 1995 Apr;31(2):314-321. doi: 10.1111/j.0022-3646.1995.00314.x